ImageJ Quick Reference Sheet

Opening an ‘.avi’ Video File:

Opening the Manual Tracking Plugin:

Possible Settings for Manual Tracking:

• For a ‘Centring Correction’: tick the box (for each object tracked); maximum = bright/white, minimum = dark/black; adjust the ‘Search square size for centring’ in the Parameters portion of the Tracking window to an appropriate value

Gathering Data with Manual Tracking:

Exporting the Manual Tracking Data: Data Window = “Results from (file name) in (units of speed)”

Challenges for Interpreting Data:

• How do we determine the correct time for a data point?

• How do we convert the X and Y pixel coordinates into x- and y-positions?

• Do we need to consider both the x and y motions? Why?/Why not?